What is RFLP (Restriction Fragment Length Polymorphism)?
The RFLP is a technique used in molecular genetics to detect genetic variations in DNA sequences. It involves the identification of the variations in the DNA sequence that alter the pattern of the DNA fragments produced by the restriction enzymes. Restriction enzymes are proteins that cut DNA at specific recognition sites. In RFLP analysis, DNA samples are digested with restriction enzymes, and the resulting fragments are separated using gel electrophoresis. Variations in DNA sequence can lead to differences in the sizes of the resulting fragments. which can be visualized as distinct banding patterns on the gel.
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Difference Between SNP and RFLP
The key differences between SNP (Single Nucleotide Polymorphism) and RFLP (Restriction Fragment Length Polymorphism), two vital genetic markers used in molecular biology. SNPs are single base pair variations in the DNA sequence, while RFLPs involve variations in DNA fragment lengths due to restriction enzyme cuts. Understanding these differences is crucial for genetic research, disease mapping, and evolutionary studies, providing insights into genetic diversity and inheritance patterns. In this article, we will study the SNP and EPLP with their similarities & differences in detail.
Table of Content
- What is SNP (Single Nucleotide Polymorphism)?
- What is RFLP (Restriction Fragment Length Polymorphism)?
- Difference Between SNP and RFLP
- Similarities between SNP and RFLP
- Conclusion: Difference Between SNP and RFLP
- Single Nucleotide Polymorphism and Restriction Fragment Length Polymorphism- FAQs