Differences Between SDS PAGE and Native PAGE
The native page vs SDS page is discussed in the following table:
|
Criteria |
SDS PAGE |
Native PAGE |
|---|---|---|
|
Description |
A technique that separates the proteins based on their molecular weight or mass |
A technique that separates the proteins based on their size, charge, and shape |
|
Feature of Gel |
Denaturing gel is used |
Non-denaturing gel is used |
|
Presence of SDS in Gel |
SDS is present |
SDS is absent |
|
Buffer composition |
The buffer has a reducing agent like DTT or BME |
The buffer has no reducing agent |
|
Sample preparation |
Protein samples are heated |
Protein samples are not heated |
|
Separation criteria |
Proteins are separated based on molecular weight |
Proteins are separated based on molecular size and overall charge |
|
Net charge on proteins |
Always negative |
Can either be positive or negative |
|
Temperature |
SDS PAGE is run at room temperature |
Native PAGE is run at 4oC |
|
Protein stability |
Unstable |
Stable |
|
State of protein |
Denatured |
Native conformation or folded state |
|
Protein recovery |
Cannot be recovered post separation |
Can be recovered post separation |
|
Protein function |
Proteins lose their function |
Proteins retain their function |
|
Used for |
To detect presence of a protein; determine molecular weight (MW) of a protein; check protein expression etc. |
To study the structure, subunit composition and function of proteins; purify proteins from a mixture in native form. |
|
Ease of use |
Easier, frequently used |
Comparatively difficult, infrequent usage |
Differences between SDS PAGE and Native PAGE
The difference between SDS-PAGE and native PAGE lies in how proteins are separated in the polyacrylamide gel. In SDS-PAGE, the migration rate of the protein is determined by its molecular weight only, whereas in native PAGE, the migration rate depends on both the size and overall charge of the protein. In this article, we will study the process of SDS PAGE and Native PAGE as well as the similarities and differences between SDS-PAGE and native PAGE.
Table of Content
- Differences Between SDS PAGE and Native PAGE
- What is SDS PAGE?
- What is Native PAGE?
- Similarities between SDS PAGE and Native PAGE
- Conclusion – Differences Between SDS PAGE and Native PAGE
- FAQs on SDS PAGE and Native PAGE