Procedure of CFT
- Sample Collection: A blood sample is collected and serum is separated from it.
- Antigen Antibody Interaction: The patient’s serum is mixed with a known antigen that is associated with the pathogen that is suspected. if the patient has antibodies against this antigen then they will bind to it.
- Complement Activation: Complement proteins are added to the mixture. if antibodies are present and bound to the antigen. then they will fix or neutralize the complements proteins.
- Indicator System: Sheep red blood cells are added as a indicator system. if the complement is not fixed due to the presence of antibodies, it will break the sheep’s red blood cells causing the visible reaction.
- Reading Results: The test results are read based on the breakage of red blood cells that occurs. if breakage is small or absent it is the positive result which means the presence of antibodies against that specific pathogen.
- Interpretation: The degree of complement presence is taken and healthcare professionals use this information to diagnose and monitor the disease.
CFT Full Form – Complement Fixation Test
CFT Full Form: CFT or Complement Fixation Test is widely used in immunological analysis which helps in the diagnosis of various infectious diseases. CFT is a highly sensitive and specific test that measures the levels of complement proteins in a patient blood serum.
CFT is widely used in the diagnosis of infectious diseases caused by bacteria, viruses, and other pathogens. Complement proteins are essential components of the immune system and their levels can provide valuable insight into the body’s response to infection. By assessing these protein levels CFT(Complement Fixation Test) assists healthcare professionals in accurately identifying and monitoring various infectious diseases which helps them to give accurate treatment plans for patients.
In this article, we have provided CFT full form, what happens in CFT with its procedure, and advantages.