Testing for Reducing Sugars
In a test tube, a combination containing Benedict’s solution (about 5 ml) and reducing sugar (about 8 drops of urine) is heated for about 2 minutes before being allowed to cool. Precipitates are produced as a result of the mixture’s color changing appropriately. This suggests fruitful outcomes.
Check the below image it shows amount of reducing sugar present depending upon different colors of solution.
Benedict’s Reagent Test Result
Test result for Benedict’s reagent are added in table below,
|
Color of Precipitate |
Concentration of Reducing Sugar (in g%) |
Interpretation |
|---|---|---|
|
Blue, No Change of Color |
0 |
No presence of reducing sugar |
|
Green Precipitate |
0.5 – 1 |
Presence of traceable amount of reducing sugar |
|
Yellow Precipitate |
1 – 1.5 |
Presence of small amount of reducing sugar |
|
Orange-Red Precipitate |
1.5 – 2 |
Presence of moderate amount of reducing sugar |
|
Brick-Red Precipitate |
>2 |
Presence of high amount of reducing sugar |
Precautions for Benedict’s Test
Some precautions while peforming Benedict’s Test are,
- Make sure to measure everything precisely
- When heating a solution, use test tube holder
- Slowly heat over a water bath. Avoid heating mixture too soon
Benedict’s Test
Benedict’s Test is a chemical test used to identify reducing sugars present in the solution. Reducing sugars are those sugars that have reducing properties. All monosaccharides and disaccharides (apart from sucrose) are reducing sugars, such as glucose, fructose, lactose, etc; they contain an aldehyde (-CHO) or a ketonic C = O. Benedict’s Test also called Benedict’s Solution or Benedict’s Reagent is used to identify simple sugars like glucose.
In this article, we will learn about Benedict’s Test, Procedure of Benedict’s Test, Preparation of Benedict’s Reagent, and others in detail.
Table of Content
- What is Benedict’s Test?
- Principle of Benedict Test
- Preparation of Benedict’s Reagent
- Procedure of Benedict’s Test
- Testing for Reducing Sugars
- Applications of Benedict’s Test
- Limitation of Benedict’s Test