What is Benedict’s Test?
Benedict’s test is a chemical test used to determine reducing sugar in any solution.
Therefore, this test can be used to identify simple carbohydrates. Benedict’s reagent, also called Benedict’s solution, is a complicated mixture of Sodium Citrate, Sodium Carbonate, and Copper(II) Sulfate Pentahydrate. Benedict’s reagent reactions result in a brick-red precipitate, when exposed to reducing sugars, indicating a positive reducing sugar test.
Benedict’s test is also used to determine whether Glucose is present in the Urine sample. As glucose is an aldose with an open chain that forms an aldehyde group and this Benedict test for urine identifies the presence of any aldehydes and α-hydroxy ketones, a positive result is obtained if glucose is present in the analyte.
Benedict’s Test
Benedict’s Test is a chemical test used to identify reducing sugars present in the solution. Reducing sugars are those sugars that have reducing properties. All monosaccharides and disaccharides (apart from sucrose) are reducing sugars, such as glucose, fructose, lactose, etc; they contain an aldehyde (-CHO) or a ketonic C = O. Benedict’s Test also called Benedict’s Solution or Benedict’s Reagent is used to identify simple sugars like glucose.
In this article, we will learn about Benedict’s Test, Procedure of Benedict’s Test, Preparation of Benedict’s Reagent, and others in detail.
Table of Content
- What is Benedict’s Test?
- Principle of Benedict Test
- Preparation of Benedict’s Reagent
- Procedure of Benedict’s Test
- Testing for Reducing Sugars
- Applications of Benedict’s Test
- Limitation of Benedict’s Test