Competitive ELISA
- It helps to measure the antigen concentration in a sample.
- The microtiter plate is coated with antigen.
- The antibody is incubated in a solution that contains antigens.
- Now add a solution of the antigen-antibody complex to the microtiter well and the wells are then washed away to remove the unbound antibodies.
- The higher the antigen concentration in the sample, the less free antibody is available to interact with the well-coated antigen.
- The enzyme-bound secondary antibody is added to detect the number of primary antibodies present in the well.
- Now the concentration can be determined through spectrophotometry.
What is ELISA? – Introduction, Procedure, Types, Applications
A basic array technique called enzyme-linked immunosorbent assay that basically used to identify and measure the antibodies, proteins, peptides, and hormones in the blood. Its test result can provide us the information about the disease that may help in planning treatment. It compares with other antibody assays to provide quantitative results and separation of non-specific and specific interactions caused by continuous binding to solid surfaces, usually polystyrene multiwell plates.
Antigen (Ag)
A toxic molecule or any foreign matter that causes an immune response in the body is called an antigen. In immunology, an antigen term originally referred to substances that generate antibodies. Antigens can be proteins, peptides, lipids, or nucleic acids.
Antibody (Ab)
An antibody is a blood protein that is produced in our body by the immune system in response to and to counteract a specific antigen. Antibodies are also called immunoglobulins.