Procedure of ELISA
ELISA is one of the easiest tests that can be done quickly and rapidly and it requires any type of sample from the patient. The entire process includes :
- Antibodies bind to polystyrene sheets, which are solid surfaces, and it was attracted to bacteria, other hormones, and antibodies.
- Antigen-coated microtiters are filled with this antigen-antibody mixture and free antibodies are removed by washing.
- A secondary antibody specific to the primary antibody is added, which normally binds to the enzyme.
- Remove free enzyme-bound secondary antibodies by washing the plate.
- Finally, the board is added. The substrate is converted by an enzyme into a colored product that can be measured spectrophotometrically.
What is ELISA? – Introduction, Procedure, Types, Applications
A basic array technique called enzyme-linked immunosorbent assay that basically used to identify and measure the antibodies, proteins, peptides, and hormones in the blood. Its test result can provide us the information about the disease that may help in planning treatment. It compares with other antibody assays to provide quantitative results and separation of non-specific and specific interactions caused by continuous binding to solid surfaces, usually polystyrene multiwell plates.
Antigen (Ag)
A toxic molecule or any foreign matter that causes an immune response in the body is called an antigen. In immunology, an antigen term originally referred to substances that generate antibodies. Antigens can be proteins, peptides, lipids, or nucleic acids.
Antibody (Ab)
An antibody is a blood protein that is produced in our body by the immune system in response to and to counteract a specific antigen. Antibodies are also called immunoglobulins.