Applications of SDS-PAGE
Few of the applications of SDS-PAGE are stated below:
- Western Blotting
- Peptide mapping.
- To measure the molecular weight of the molecules.
- To estimate the protein size.
- To analyze post-translational modifications.
- To compare the polypeptide composition of different structures.
- Protein ubiquitination.
- To check the protein purity.
- To analyze the size and number of polypeptide subunits.
- To separate the HIV proteins in the HIV test.
SDS-PAGE
SDS PAGE, or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis, is a technique for separating proteins depending on their molecular weight. SDS is an anionic surfactant and detergent. SDS breaks down the non-covalent links of protein molecules. The method of separating protein molecules according to their electrophoretic mobility is frequently used in molecular biology, genetics, forensics, and biotechnology. In this article, we will read about SDS-PAGE, its principles, its methods, and procedures required to carry out its process, and its applications.
Table of Content
- Define SDS-PAGE
- What is Electrophoresis?
- What is SDS-PAGE?
- Principle of SDS-PAGE
- Materials Required
- Procedure of SDS-PAGE
- Applications of SDS-PAGE
- Importance of SDS-PAGE