What is SDS-PAGE?
Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis is referred to as SDS-PAGE. Proteins can be separated and analysed using this commonly used laboratory approach according to their size. For characterizing protein samples, determining their molecular weights, and evaluating purity, SDS-PAGE is especially helpful. The approach requires the use of a polyacrylamide gel matrix and the detergent sodium dodecyl sulphate (SDS) to denature and linearize proteins.
SDS-PAGE
SDS PAGE, or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis, is a technique for separating proteins depending on their molecular weight. SDS is an anionic surfactant and detergent. SDS breaks down the non-covalent links of protein molecules. The method of separating protein molecules according to their electrophoretic mobility is frequently used in molecular biology, genetics, forensics, and biotechnology. In this article, we will read about SDS-PAGE, its principles, its methods, and procedures required to carry out its process, and its applications.
Table of Content
- Define SDS-PAGE
- What is Electrophoresis?
- What is SDS-PAGE?
- Principle of SDS-PAGE
- Materials Required
- Procedure of SDS-PAGE
- Applications of SDS-PAGE
- Importance of SDS-PAGE