What is Electrophoresis?
A laboratory procedure called electrophoresis is used to separate and examine molecules according to their size and charge, including proteins, DNA, RNA. The process is based on charged particles moving through a medium—usually a gel in an electric field. There are several kinds of electrophoresis, such as DNA, RNA, and protein electrophoresis, each designed for certain macromolecules and applications.
The fundamental idea is to pass an electric current through a gel or other supporting material that holds the relevant sample. Due to their charge, the molecules in the sample migrate across the medium at different speeds based on their size and charge. Larger or fewer negatively charged molecules travel more slowly, whereas smaller and more negatively charged molecules move faster.e.g.: Gel electrophoresis, paper electrophoresis, immunoelectrophoresis, etc.
SDS-PAGE
SDS PAGE, or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis, is a technique for separating proteins depending on their molecular weight. SDS is an anionic surfactant and detergent. SDS breaks down the non-covalent links of protein molecules. The method of separating protein molecules according to their electrophoretic mobility is frequently used in molecular biology, genetics, forensics, and biotechnology. In this article, we will read about SDS-PAGE, its principles, its methods, and procedures required to carry out its process, and its applications.
Table of Content
- Define SDS-PAGE
- What is Electrophoresis?
- What is SDS-PAGE?
- Principle of SDS-PAGE
- Materials Required
- Procedure of SDS-PAGE
- Applications of SDS-PAGE
- Importance of SDS-PAGE