SDS-PAGE
What is SDS-PAGE?
SDS-PAGE is an electrophoretic technique used to separate protein molecules based on their molecular weight.
Why is SDS added to Polyacrylamide gel?
The SDS breaks the disulfide bonds and modifies the protein structure. These modified structures and increased negative charge help to maintain the charge-to-mass ratio in electrophoresis.
Which Stain is used in SDS-PAGE?
To stain peptide bonds in the sample the Coomassie blue stain is used in SDS-PAGE. It stains the peptide bond blue.
What is TEMED?
TEMED is also known as Tetramethylethylenediamine. It is an ethylenediamine derivative.It is commonly used for polyacrylamide gel preparation in combination with ammonium persulfate.
What is Polyacrylamide?
Polyacrylamide is a synthetic linear polymer which is composed of acrylamide or a mixture of acrylamide and acrylic acid. It is water-soluble and used in pulp and paper industries.
What is the Function of SDS in Protein Extraction?
SDS is an anionic detergent that disintegrates the covalent bonds between the proteins and confers the negative charge to the protein according to its mass.
Which Buffer is used in SDS-PAGE?
The buffer used in SDS-PAGE is the SDS running buffer that is made up of Tris-HCl, SDS, glycerol, beta mercaptoethanol (BME) and Bromophenol Blue.
SDS-PAGE
SDS PAGE, or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis, is a technique for separating proteins depending on their molecular weight. SDS is an anionic surfactant and detergent. SDS breaks down the non-covalent links of protein molecules. The method of separating protein molecules according to their electrophoretic mobility is frequently used in molecular biology, genetics, forensics, and biotechnology. In this article, we will read about SDS-PAGE, its principles, its methods, and procedures required to carry out its process, and its applications.
Table of Content
- Define SDS-PAGE
- What is Electrophoresis?
- What is SDS-PAGE?
- Principle of SDS-PAGE
- Materials Required
- Procedure of SDS-PAGE
- Applications of SDS-PAGE
- Importance of SDS-PAGE