Principle of SDS-PAGE
A charged molecule when placed in an electric field migrates to the oppositely charged electrode. The charged molecules get separated based on the relative mobility of charged species. The less electrophoretic resistance in faster migration of smaller molecules. The protein structure and the charge on it also influence the migration rate. SDS and polyacrylamide remove the impact of protein structure and charge. It separates the protein based on the length of the polypeptide chain.
SDS-PAGE
SDS PAGE, or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis, is a technique for separating proteins depending on their molecular weight. SDS is an anionic surfactant and detergent. SDS breaks down the non-covalent links of protein molecules. The method of separating protein molecules according to their electrophoretic mobility is frequently used in molecular biology, genetics, forensics, and biotechnology. In this article, we will read about SDS-PAGE, its principles, its methods, and procedures required to carry out its process, and its applications.
Table of Content
- Define SDS-PAGE
- What is Electrophoresis?
- What is SDS-PAGE?
- Principle of SDS-PAGE
- Materials Required
- Procedure of SDS-PAGE
- Applications of SDS-PAGE
- Importance of SDS-PAGE