Materials Required
Material used in SDS-PAGE: SDS-PAGE requires samples, gels, staining and destaining buffers or dyes, running buffers, power supplies, and a reference ladder.
Material |
Role |
---|---|
Protein/Samples |
To check the purity. |
Gels |
Smooth migration of sample. |
Electrophoresis Chambers |
For fitting the SDS-PAGE. |
Electrophoresis Chambers |
Migration of sample in the gel. |
Staining and Destaining Buffer |
To make the sample visible. |
Power Supplies |
Convert the AC to DC. |
Reference Protein Ladder |
To locate the desired protein from the sample. |
SDS-PAGE
SDS PAGE, or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis, is a technique for separating proteins depending on their molecular weight. SDS is an anionic surfactant and detergent. SDS breaks down the non-covalent links of protein molecules. The method of separating protein molecules according to their electrophoretic mobility is frequently used in molecular biology, genetics, forensics, and biotechnology. In this article, we will read about SDS-PAGE, its principles, its methods, and procedures required to carry out its process, and its applications.
Table of Content
- Define SDS-PAGE
- What is Electrophoresis?
- What is SDS-PAGE?
- Principle of SDS-PAGE
- Materials Required
- Procedure of SDS-PAGE
- Applications of SDS-PAGE
- Importance of SDS-PAGE